Search results for " S-RNase"
showing 2 items of 2 documents
Intra-allelic variation in introns of the S13-RNase allele distinguishes sweet, wild and sour cherries
2010
The cherry (Prunus avium), a self-incompatible diploid species, and the sour cherry (Prunus cerasus), a self-incompatible or self-compatible allotetraploid species derived from P. avium and Prunus fruticosa, share several S-RNase alleles, including S13. An inactive form, S13° ,i s found in some sour cherries. Two (AT) microsatellites are associated with allele S13-RNase, one in the first intron and one in the second. Their length polymorphisms were studied in 14 sweet and 17 wild cherries (both P. avium) and in 42 sour cherries. Fluorescent primers amplifying each microsatellite were designed and amplification prod- ucts sized on an automated sequencer. Variants ranged from 247 to 273 bp fo…
Analysis of S-allele genetic diversity in Sicilian almond germplasm comparing different molecular methods
2015
Italian almond germplasm is characterized by a wide diversity in several growing areas among which Sicily is one of the most important. Analysis with consensus and specific primers and DNA sequencing was performed to investigate S-RNase genetic diversity and to elucidate the homology rate within a genetic pool of 27 Italian accessions. Interestingly, some of the self-compatible cultivars did not show the presence of Sf allele. Amplicons from consensus and allele-specific PCR primers revealed a high level of variability. Sequencing of all the S-RNase amplicons derived from consensus primers allowed the identification of two new S-RNase alleles (S51 and S52). Surprisingly, despite the AA repl…